| Prague State Opera | | | |
| Vincenzo Milletarì | Conductor | | |
| Jiří Štrunc | Conductor | | |
| Jiří Heřman | Director, Set Designer | | |
| Alexandra Grusková | Costume Designer | | |
| Jan Lukášek | Set Designer | | |
| Daniel Tesař | Lighting Designer | | |
| Prague State Opera Chorus | | | |
| Prague State Opera Orchestra | | | |
| Olga Busuioc | Soprano | Cio-Cio-San (Madama Butterfly) | |
| Maria Kobielska | Soprano | Cio-Cio-San (Madama Butterfly) | |
| Peter Berger | Tenor | Pinkerton | |
| Luciano Mastro | Tenor | Pinkerton | |
| Pavol Remenár | Baritone | Sharpless | |
| Svatopluk Sem | Baritone | Sharpless | |
| Štěpánka Pučálková | Mezzo-soprano | Suzuki | |
| Jana Sýkorová | Mezzo-soprano | Suzuki | |
| Michaela Zajmi | Mezzo-soprano | Suzuki | |
| Miloš Horák | Bass | The Bonze | |
| Jiří Přibyl | Baritone | The Bonze | |
| Igor Loškár | Baritone | Prince Yamadori | |
| Matúš Mazár | Baritone | Prince Yamadori | |
| Josef Moravec | Tenor | Goro | |
| Václav Sibera | Tenor | Goro | |
| Oleg Korotkov | Bass | The Imperial Commissioner | |
| Roman Vocel | Bass | The Imperial Commissioner | |
| Libor Novák | Bass | The Official Registrar | |
| Peter Paleček | Baritone | The Official Registrar | 2020 Jan 16, 21, 26, 30, Feb 16, 20, Mar 21, 29 |
| Lenka Pavlovič | Soprano | Kate Pinkerton | |
| Erika Vocelová Jarkovská | Mezzo-soprano | Kate Pinkerton | |
| Tomáš Foltýnek | Bass | Yakuside | |
| Michael Skalický | Baritone | Yakuside | |
| Soňa Koczianová | Soprano | Cio-Cio-san's mother | |
| Markéta Frýdová | Mezzo-soprano | Cio-Cio-san's mother | |
| Gabriela Pešinová | Soprano | The aunt | |
| Eliška Sedláčková | Soprano | The aunt | |
| Eliška Mourečková | Soprano | The cousin | |
| Tereza Růžičková | Soprano | The cousin | |